字典翻译 问答 小学 英语 英语翻译InatypicalplasmidtransformationexperimenttherewillbeanextremelyheterogeneousmixtureofDNAmoleculeswithonlyasmallnumberconsistingofasinglevectormoleculelinkedtoasingleDNAfragment.WhenE.coliisusedfortrans
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英语翻译InatypicalplasmidtransformationexperimenttherewillbeanextremelyheterogeneousmixtureofDNAmoleculeswithonlyasmallnumberconsistingofasinglevectormoleculelinkedtoasingleDNAfragment.WhenE.coliisusedfortrans
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英语翻译

InatypicalplasmidtransformationexperimenttherewillbeanextremelyheterogeneousmixtureofDNAmoleculeswithonlyasmallnumberconsistingofasinglevectormoleculelinkedtoasingleDNAfragment.WhenE.coliisusedfortransformationtheDNAmoleculesaremixedwiththebacterialcellsthathavebeenmadecompetentorpermeablebybeingexposedtoacoldsolutionofcalciumchloride(0.1M).Thecellsarethenwarmedto37℃for5minutestoallowtheDNAtobetakenup.ThecellsarethentransferredtobrothandafterashortincubationperiodareabletoexpressthefunctionsofthenewlyacquiredchimericDNA.Thebacteriacarryinghybridmoleculesareidentifiedbyaspecificscreeningprocedureandpurified:theirhybridplasmidsareisolatedandcharacterized,orthenewproductsareisolatedandstudied.Transformationefficienciesareabout10^6transformantsperμgcloningmixtureDNA.

陈志坚回答:
  靠这么简单的东西还要翻啊?你水平太差了
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